volumes with methanol .After removal of the plates, allow them to d'ry in air and examine under ultra-violet light (254 nm). Any secondary spot

volumes with methanol .After removal of the plates, allow them to d'ry in air and examine under ultra-violet light (254 nm). Any secondary spot in the chrornatograms obtained with solution (1) is not more intense than the spot in the chromatogram obtained with solution (3) and not more than two such spots are more intense than the spot in the chromatograms obtained with solution (5) Ignore any spot remaining at the point of application.Sulphated ash: Not more than 0 .1 %, Appendrx 3.22.Loss on drying not more than 1 .0%, determined on 1 g by drying in an oven at 105° for 3 hours. Appendix 8.6 . Assay Weigh accurately about 0. 3 g. dissolve in 50 ml of ethanol (95%) and add 1 ml of 0. 1M hydrochloric acid Carry out the method for potentiornetric titration, Appendix 3. 47, using 0.1 M sodium hydroxide as the titrant Read the volumes added between the two points of inflection. Each mi of 0.1M sodium hydroxide isequivalent to 0. 03729 g of C18H28N2O4 .HCLACEBUT0L0L HYDRO CHLORIDE TABLETSAcebutOlol TabletsUsual strengths 200 mg, 400 mg .Storage Store in tightly-closed, light-resistant containers STANDARDSAcebutolol Hydrochoride .Tablets contain not less than 95.0 percent and not more than 105.0 per cent of the stated amount of acebutolol hydrochloride, C18 H28 N2 04 HCL. The tablets may be coated .Identification; A The light absorption in the range 220 to 360 nm of the solution obtained in the Assay exhibits a mawmurn at about 233 nrn. Appendix 5 5.B Comply with test C described under Acebutolol Hydrochlonde but using as soultion [1) a solution prepared in the following manner. Shake a quantity of the powdered