solution in 0 .1% v/v solution of hydrochloric acid exhibits two maxima, at about 233 nrn and 322 nm, absortance at about 233 nrn between

solution in 0 .1% v/v solution of hydrochloric acid exhibits two maxima, at about 233 nrn and 322 nm, absortance at about 233 nrn between 0 .55 and 0 .61, Appendix 5. 5. C Carry out the method for thin-layer chromatography, Appendix 4. 6. using silica gel GF254 as the coating substance and a mixture of 60 volumes of water, 40 volumes of methanol and 0. 5 volume of perchloric acid as the mobile phase. Apply separately to the plate 10 ul of each of the following solutions in methanol Solution (1) contains 0.1%w/v of the substance being examined. Solution (2) contains 0.1 % w/v of acebutolol hydrochlonride RS. Solution (3) is a mixture of equal volumes of solution (2) and a solution of 0. 1 % w/vof pindofol RS After removal of the plate, allow it to dry in air and examine under ultra-violet light (254 nm). The principal spot in the chromatogram obtained with solution (1) corresponds to that in the chromatogram obtained wrth solution (2) The test is not valid unless the chromatogram obtained with solution (3) shows two clearly separated spots D; A 5% w/v solution gives reaction A of chlorides. Appendix 3 .1.E Melts at about 143°, Appendix 8.8 pH Between 5.0 and 7.0, determined in a 1. 0% w/v solution. Appendix 8.11.Clarity and colour of solution. A 5% w/v solution is not more opalescent than opalescence standard OS2, Appendix6 1. and not more intensely coloured than reference solution BYSS, Appendix 6 2 Heavy metals Not more than 20 ppm, determined on 1 ,0 g by Method A, Appendix 3. 12 .Related substances Carry out the method for thin-layer chromatography. Appendix 4. 6b using silica gel GF254 as the coating substance on two plates, the upper layer obtained by shaking together 50 volumes of water. 40 volumes of 1-butanol and 10 volumes of glacial acetic acid as the mobile phase A and a mixture of 90 volumes of 2-propanol and 10 volumes of glacial acetic acid as the mobile phase B. Apply separately to the plates 20 ul of each of the following solutions in methanol. Solution (1) contains 1.0% w/v of the substance being examined .For solution (2) dilute 1 ml of solution (1) to 10ml. For solution (3) dilute 3 ml. of solution (2) to 10O ml. Solution (4) is a 0. 1% w/v solution of acebutolol hydrochloride RS. For solution (5) dilute 1 volume of solution (4) to 3