Cool and add 5M sodium hydroxide. the colour becomes yellowish-blown. Related substances. Carry out the test method described under Bisacodyl Supposi

solution A with a few drops of nitric acid, a yellow colour is produced. Cool and add 5M sodium hydroxide. the colour becomes yellowish-blown. Related substances. Carry out the test method described under Bisacodyl Suppositories using as solution (1) a solution prepared in the following manner Shake a quantity of the powdered tablets equivalent to 20 mg of Bisacodyl with 2 ml of acetone for 10 minutes, centrifuge, and use the supernatant liquid. Any secondary spot in the chromatogram obtained with solution (1), other than any spot (due to tablet excipient), having a relative Rf of 1.3 compared with the principal spot, is not more intense than the spot in the chromatogram obtained with solution (2). Uniformity of content. Comply with the requirements stated under Tablets using the following method of analysis. Powder one tablet, shake with 70 ml of chloroform for 30 minutes and dilute with sufficient chloroform to produce 100 ml. Mix well, filter and discard the first few ml of the filtrate. Measure the absorbance of the filtrate at the maximum at about 264 nrn, Appendix 5.5. Calculate the content of C22H18N04 taking 148 as the value of A(1 %, 1 cm) at the maximum at about 264 nm. Disintegration Comply with the requirements stated under Tablets using a 1 5% w/v solution of sodium bicarbonate in place of mixed phosphate buffer pH 6.8. Other requirements Comply with the requirements of tests stated under Tablets Assay. Weigh and powder 20 tablets. Weigh accurately a quantity of the powder equivalent to 40 mg of Bisacodyl, shake with 70 ml of chloroform for 30 minutes, dilute to 100 0 ml with chloroform, mix, filter and dilute 10 ml of the filtrate to 100 ml with chloroform. Measure the absorbance of the resulting solution at the maximum at about 264 run. Appendix 5.5.